We narrowed to 580 results for: SIM
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TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... and interested in science. “Postdoc hires are simpler because of their documented history in science....
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From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....resource: connecting with mentors who have made similar transitions can provide you with insights, lessons... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....it as GFP+. Quantification for such graphs could simply be the percentage of the total cells present in... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error prone PCR, EvolvR uses... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....scientists around the world. In 2004, that was a fairly simple goal… and we had no idea how much our understanding... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....at their core they can all be boiled down pretty simply – they are systems that measure biological phenomena... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....closely related, their required components are very similar. While plasmids containing virus-specific elements... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....the brain to the rest of the body. Therefore, by simply looking at how neurons are physically connected... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....Libraries allow you to test thousands of elements simultaneously in your system instead of just one at a time... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....fused fluorescent protein. This technique is very similar to the widely used FRET, but it eliminates the ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....The notion of cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....Further, the program takes this into account when simulating a digest as depicted in Lane 2. Controlling ... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m.... for a local school’s Science Department, or to simply tour the lab as a starting point into further STEM... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....for either tissues or cells. The process is very similar for both, though tissue samples do require an extra... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....response in a certain cell type. Chemogenetics is similar to optogenetics but instead of light, these genetically... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....allows for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells...