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...outlining the different steps and materials required for each [1, 2]. This protocol [3] provides, in exquisite... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...the laser must be controlled. As discussed above, each opsin variant has slightly different activation/...

...longer term and more substantial experience can be achieved by volunteering for a professional organization...organized a “Committee Leadership Institute” (CLI) where each Committee Chair and Vice Chairs were invited to ...was ultimately invited to join the Advocacy and Outreach Committee within a professional society in my ...

...markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table ...future in. And we decided to make education and outreach a cornerstone of our business, because we are ...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

...confluent because cells like their space. If they reach confluence, they stop growing (contact inhibition.... Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent...pipettes for liquid waste) should be sterilized before each use. Also, all contaminated liquid and solid waste...

...pBAD vectors for example. Protein expression from each of these vectors is controlled by a different promoter...resulting in different levels of expression from each vector; lower expression may be required if your...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...

...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...releasing the virus to the supernatant. The virus will reach peak production when roughly 60–70% of cells have...

...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...it is critical to include an unstained sample for each tissue type you are running in your panel if you...

... current yellow fluorescent proteins tend to photobleach rapidly, which hinders their use for long-term...Alejo Rodriguez-Fraticelli recently developed an approach called Simultaneous Tracking of Recombinase Activation... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...

...Addgene AAV Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation...immediately before iodixanol purification. This approach gives me a bit of flexibility since I can store... Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and purification! References...

... through the specimen, which often leads to photobleaching.  Furthermore, point-by-point scanning along...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...215. Pubmed. W. Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten...

... Cas9-gRNA ribonucleoproteins? One alternative approach, which avoids many of these complications, is ...vendor, this can often be expensive. An alternative approach is to express and purify His-tagged Cas9 from ...inner ear (Zuris et al. 2015). A more targeted approach using RNPs has recently been described in vitro...

...labels (green circles in figure 2 below) can be attached to each antibody. But being big is a double-edged... to different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site ...

...that you will contribute openness, clarity, approachability, and professionalism to team meetings and projects...second to reflect. For example, say your boss approaches your team with a project she needs one of you...to produce such great work. Say your manager approaches you with glowing feedback from a client you helped...

...information, you can fuse each FP fragment to the N- and C-terminal end of each PIP and empirically test...practice because FRET depends on a number of hard-to-achieve factors. For example, FRET requires that fusion...

...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

... and coach you. There are many grant consultants who offer grant training, mentoring and coaching services...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant ...

...regions of interest in a non-biased manner. To achieve this goal, it is necessary to isolate specific ...speakers. They made an every effort to interact with each other, which is critically important, especially...slow, but if they don't stop, they may be able to achieve something that may change the rules of the game...

...reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria ...coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene is present in the donor vectors ...toxic to the cell, Gateway cloning efficiency can reach >99%.   STEP 3: Express your Gene of Interest...

...techniques are used as part of a treatment plan, an approach known as multimodal therapy. Sadly, in many cases...the abdominal cavity. This targeted chemotherapy approach has been shown to be successful in killing many...as in the case of Paul Zygielbaum. A different approach to immunotherapy is bevacizumab (Avastin). This...

...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...circuits are organized and how neurons connect to each other). Combined with AAVs, FPs can be easily expressed...