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We narrowed to 816 results for: ache

Showing: 441 - 460 of 816 results

  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ... Cas9-gRNA ribonucleoproteins? One alternative approach, which avoids many of these complications, is ...vendor, this can often be expensive. An alternative approach is to express and purify His-tagged Cas9 from ...inner ear (Zuris et al. 2015). A more targeted approach using RNPs has recently been described in vitro...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...labels (green circles in figure 2 below) can be attached to each antibody. But being big is a double-edged... to different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site ...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...that you will contribute openness, clarity, approachability, and professionalism to team meetings and projects...second to reflect. For example, say your boss approaches your team with a project she needs one of you...to produce such great work. Say your manager approaches you with glowing feedback from a client you helped...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... and coach you. There are many grant consultants who offer grant training, mentoring and coaching services...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant ...

  5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...regions of interest in a non-biased manner. To achieve this goal, it is necessary to isolate specific ...speakers. They made an every effort to interact with each other, which is critically important, especially...slow, but if they don't stop, they may be able to achieve something that may change the rules of the game...

  6. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...genetic manipulation still need to be developed for each organism, but researchers are optimistic that this... providing support "for development of tools, approaches, and infrastructure necessary for direct tests...said they were hard to raise because they beat on each other in captivity. Sticklebacks demonstrate unusual...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria ...coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene is present in the donor vectors ...toxic to the cell, Gateway cloning efficiency can reach >99%.   STEP 3: Express your Gene of Interest...

  8. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...techniques are used as part of a treatment plan, an approach known as multimodal therapy. Sadly, in many cases...the abdominal cavity. This targeted chemotherapy approach has been shown to be successful in killing many...as in the case of Paul Zygielbaum. A different approach to immunotherapy is bevacizumab (Avastin). This...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...circuits are organized and how neurons connect to each other). Combined with AAVs, FPs can be easily expressed...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems. The original pCas/pTargetF...dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that is 2.6 times... gene (cluster) interruption.     Plasmids for each of these systems are available from Addgene. If ...

  11. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...high-level career advice, open doors for you, and teach you valuable lessons along the way. We think it’...physically walking through the protocol, by going to each space in order to double-check you remember where...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ... types of ELISA, advantages and disadvantages of each, and some considerations when using the method. ...non-overlapping epitopes so that they do not interfere with each other. The detection antibody may either be conjugated... Overview of the advantages and disadvantages of each ELISA method Method Advantages Disadvantages...

  13. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... at the requirements in the job description. For each requirement, ask yourself if you have that skill...greeting like “Dear Hiring Manager.” Highlight your achievements with specific examples. Instead of saying “I...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... plasmids to utilize diverse host replication machinery. For instance, the ori typically contains iterons...However, if plasmids have their own replication machinery such as Rep or other initiator proteins, they ...Rhizobium spp. IncP RK2 Gram-negative  Achromobacter parvulus, Acinetobacter spp., Aeromonas spp....

  15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...strands must be available in moderate proximity to each other.  If having two sgRNAs isn’t feasible for your...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

  16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to...the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance runner, gardner...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...the technique and ask if they would be willing to teach you. An essential, yet sometimes overlooked, aspect...transgenic rat technology is improving and may one day reach the sophistication of mouse transgenics. Everything...[10]. If used properly, optogenetics is sure to teach us much in all of these species. Happy experimenting...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...typically want to make sure your cells have first reached an appropriate density. Cells in the exponential...protein products, which can prevent the culture from reaching the desired density within a reasonable time-frame...cystosolic protein products, but this is not always achievable. Proteins which contain hydrophobic regions or...

  19. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...sciNote. Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases...familiar paper-based lab notebook is still a standard approach to record-keeping in many labs. In a way, it will...digital data generated in labs. While many labs approach the situation differently, some adopting simple...
Showing: 441 - 460 of 816 results