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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 804 results for: arc

Showing: 441 - 460 of 804 results

  1. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...devising their tagging strategy, another group of researchers was working on isolating monoclonal antibodies...identified oncogenes and was a target of intense research. Evan, et al. hoped that their new monoclonals...producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable domains of Myc1-...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior...commonly administered through the tail vein — many researchers will simply refer to these as “tail vein injections...feeding tube can be flexible or metal, depending on researcher preferences. It sounds complicated but has a ...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...plasmid encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... essential part of being able to continue your research. As an academic postdoc, you’re often required... at once at the end of February or beginning of March, and the jobs are mixed into the general careers...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

  6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre Marillonnet share...and since we are making all the tools for plant research here and validating them, it made the most sense...academic in literature who gave a talk to the women’s research network here. She talked about the advantages ...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...variants, and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many ... & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor...to Our Guest Blogger! Gal Haimovich, PhD, is a research fellow in the lab of Prof. Robert Singer at Albert...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...delivered using this vector. For example, although researchers know that Usher Syndrome (a disease that results...encouraging. Importantly, a significant amount of basic research is being done to further improve the technology...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...accessibility (Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural...Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses... microscopy: principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...they’re seeking someone who has, to a t, all the research skills under your belt from your last job. You...managing inventories in the lab, or organizing a research project, one can assume that you’ve had to flex...

  11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...Lavis is currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would...

  12. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance...’re turning the tables on the bacteria. Future research will show if this new strategy can help us win... on Addgene.org Find CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced... Marcy Patrick...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...

  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...

  20. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...
Showing: 441 - 460 of 804 results