We narrowed to 533 results for: erf
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TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....PMCID: PMC4777285. Further Reading Microscopy U. Generfal Considerations when Using Fluorescent Proteins...
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Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....isolate particular organelles and their contents, or perform in situ hybridization experiments. In many cases... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m.... Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....hundreds of nanograms of DNA, you will likely need to perform several individual transformations, such as splitting... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....corrected flow plot in 2A, the populations are not perfectly straight, but the center of each population is... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....starving. I think plant biology is massively underfunded considering how important it is. I would really... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....should be free to use and used for good. It's wonderful to have Addgene as a professional public-benefit... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m.... screens they analyzed were designed to have a perfect match to the reference genome – even single mismatches... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m...., Diaz MFP, Yong WH (2018) An Introduction to Performing Immunofluorescence Staining. Methods in Molecular... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....students each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m.... PCR/sequencing While CRISPR-transposons are powerful and simple to use, users should keep aware of ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m.... the cell’s nucleus. But since nothing’s ever perfect, neither is AAV. Its main limitation is the small... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....trendline. R2 ranges from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors for neurons,... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...