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We narrowed to 782 results for: ina

Showing: 441 - 460 of 782 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...It’s through the PTA that I recently started coordinating the annual Math & Science Night, a chance for...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...

  4. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...

  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...template control PCR reaction for detecting contamination is also a good idea.    Analyzing PCR product...positive clones (+) compared to negative clones. Finally, orientation-specific primers (3) give the same...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...virulent or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria...including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics by their suppliers, and... DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which may also induce cell...

  8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...signal sequence (gene start) and a transcription termination/polyadenylation signal sequence (gene end). A...encoded in separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers...it reaches the gene end signal, where it will terminate transcription and polyadenylate the mRNA. These...

  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...isoforms of these proteins, as well as the viral long terminal repeats (LTRs) are different. As a result, lentiviral...modified the 5’ LTR of the transfer plasmid to eliminate the need for the HIV Tat protein and consequently...initial infection. SIN is shorthand for self-inactivating, which is achieved by deleting a large portion...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis...mesothelioma tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the ...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...

  12. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...concentration.  Screening time Phew! You are now (finally!) ready to use your transduced cells to perform...

  13. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...sequences each week. Our internal software was originally designed to help us work in large formats, such...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...

  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating as they are good at...Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. ...

  15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes...JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...

  16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...mice is likely due to how the Deverman and the Gradinaru labs engineered PHP.B. PHP.B was created by first...suggested there was a single gene that had two codominant versions or alleles that was driving the PHP.... available from Addgene. References Holmes, Christina, and William L. Stanford. "Concise review: stem...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to induce gene...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely...develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s a natural .... "Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly important...find my tips for a perfect science talk here). Finally, my main content organizer is the amazing app Evernote...
Showing: 441 - 460 of 782 results