We narrowed to 486 results for: mme

1. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...different. Some of these changes you may enjoy immediately and some of them may just take some getting used...

2. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...the PAM sequence. Avoid targeting a ‘C’ placed immediately after a ‘G’ base because CBE is inefficient in...

3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death." Proceedings of the National...

4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...with all plasmids in the repository, we highly recommend reading the associated publications or protocols...

5. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...

6. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial...

7. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

8. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...should validate it for your particular setup. We recommend starting by searching the literature to see what...

9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...tolerate a mismatch in the binding sequence, we recommend changing several bases as close to the PAM as ...

10. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...

11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

12. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...performance. By this point, towards the end of the summer, things were getting better and the office had ...

13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...tagging vectors for your specific gene, the lab recommends designing homology arms with the modification...

14. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ... cut vector with excellent efficiency using a commercial isothermal assembly master mix. Introduction ...

15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ...10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ...

17. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

18. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...system. As we cannot make universal serotype recommendations, the following table focuses on tissue tropism...

20. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...