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We narrowed to 689 results for: mos

Showing: 441 - 460 of 689 results
  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can...publication, including the supplemental data. You’re most likely to find thorough characterization data, like...
  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...several ways to achieve multiplex IF. One of the most common approaches is to use sequential cycles of...are a few specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies...
  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing system derived... conventional methods. First, one can work with almost any strains of mice, as compared with conventional...
  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...
  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...expression in the retina and in parts of the brain, but almost none in the liver (A). In general, RNA levels tend...analyze the resulting data, so it may not be the most accessible option for many laboratories. Additionally...
  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... is a powerful experimental strategy, one of the most common concerns we get here at Addgene about this...-homologous pairs) (Figure 2.). They found that almost all off-target expression can be attributed to ...
  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...Using a DNA template for the RNAi trigger is the most prevalent method due to ease of use and capacity...actively silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly ...
  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...recognize the same epitope of the target protein. Most commonly these antibodies are produced from hybridomas...fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors...
  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...
  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...many applications in biotech, and is considered the most studied Gram-positive bacterium, many of its genes...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops...
  11. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses...
  12. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...
  13. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to...
  14. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers...
  15. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...conference blog posts before you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow...
Showing: 441 - 460 of 689 results