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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 745 results for: nes

Showing: 441 - 460 of 745 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier...2P has faster response kinetics and increased brightness, sensitivity, and photostability under two-photon...screen, selected for increased voltage response, brightness, and photostability. It enables sustained deep-tissue...

  2. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...are amenable to genetic manipulation, nor can all genes be easily knocked down or out.   Example: You just...data that convinced you of your antibody’s trustworthiness. You may also consider sharing your validation..., Anderson AP, Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms which...several aspects of editing. First, they devised guidelines for designing single-stranded repair templates...on gene regulation in nematode molting and spermatogenesis. His group also builds tools to improve genome...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...transition Base changes between purines (A ↔ G) or between pyrimidines (C ↔ T).   Base transversion  ...conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using...

  5. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...Created by Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at...at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford...

  6. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...blog posts showcasing the work of depositors) Wednesday: Why deposit? Thursday: Throwback Thursday (Blue...

  7. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...AAV9 is so strong that you can even see dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  We love negative...

  10. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can ...of published and commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include...

  11. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...library (~60,000 gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs...APOBEC1 causes genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE ...

  12. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at...to generate the legal agreement (MTA), which streamlines the process and saves you time. Anyone affiliated...

  13. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...biggest changes, there are plenty of less obvious ones to discover as you browse. You may find one of your...choices informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check...

  14. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and...Most chromatogram viewing programs (even the free ones) allow you to edit the sequence.   I hope these...

  15. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative...

  16. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian...

  17. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start something... publications like FierceBiotech to “learn the business” Attend events with local networking and trade...

  19. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...
Showing: 441 - 460 of 745 results