We narrowed to 507 results for: otos

1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds in the well...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...reproducibility. Non-recombinant antibodies are notorious for being the source of irreproducible data (Begley...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...

4. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional...

5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...sfCherry2 sfCherry2(1-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10) Improved split...

6. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...Educational Resources Sequence Analysis of a Plasmid Protocol ...

7. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...overhang specific to the type of module it is (Pol 2 protomer, Cas protein, ect...). Whatever individual components...

9. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583–1601. doi: 10.1038/nprot.2013.094....

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

11. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...spatiotemporally controllable version of the BioID protocol. The critical component for both BioID and Split-BioID...

12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative ...

13. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

14. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–3616. https...

15. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...

17. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

18. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ..., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo...

19. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...and execution of CRISPR in vivo screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...