We narrowed to 509 results for: otos

...explains 1-2 potential pitfalls of the intended protocol, and offers alternative solutions to demonstrate...

...and encourage you to check out the lentiviral protocols and blog posts on Addgene’s website for additional...

...generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583–1601. doi: 10.1038/nprot.2013.094....

...Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

...spatiotemporally controllable version of the BioID protocol. The critical component for both BioID and Split-BioID...

...and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative ...

...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

...Educational Resources Sequence Analysis of a Plasmid Protocol ...

...overhang specific to the type of module it is (Pol 2 protomer, Cas protein, ect...). Whatever individual components...

...sfCherry2 sfCherry2(1–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split...

...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...

...protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds in the well...

...reproducibility. Non-recombinant antibodies are notorious for being the source of irreproducible data (Begley...

...knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038/nprot.2017.016...

...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...

...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...