We narrowed to 460 results for: try
-
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc...
-
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....neurons: differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....Single Chain Fv Protein." Journal of Biological Chemistry (2000). PubMed PMID: 10748019. 8. Lotze, Jonathan... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....effective, in both academic circles as well as in industry. The news is good. In their ideal form, ELNs provide... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....characterization of dedicated sensors for FLIM, for ratiometry and with high affinity." PloS one 10.4 (2015)... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897–17901. https://doi.org/10.1074... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....’s headquarters, the skies are getting dark and wintry with the end of year approaching. We’re always ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....matrix metalloproteinases." Journal of Biological Chemistry 290.8 (2015): 4728-4740. You can find more info... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....and high throughput state detection with flow cytometry. However, expressing reporters will consume cellular... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....marker can be separated by an internal ribosome entry site (IRES) and expressed under the same promoter... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....work (now recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their ... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m.... the most complex prokaryotic immune system. Biochemistry (Moscow), 86(10), 1301–1314. https://doi.org... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....fluorescent protein into a photoactivatable probe." Chemistry & biology 12.3 (2005): 279-285. PubMed PMID:15797211... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....understanding of cellular mechanisms. Advancements in dye chemistry have led to the development of more stable, brighter... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....transgenic mice for optogenetic dissection of neural circuitry function. Nature methods. 2011;8(9):745-52. PMID... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....recommend doing a little more research into gel chemistry and/or specialty gel options. Table 1: Types of... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....your strain of interest, check the mechanism of entry if known, and, as always, validate the capsid for...