We narrowed to 599 results for: REV;

1. Let There Be LITE Plasmids

   Type

   Blog Post

   Published

   Nov. 21, 2013, 3:56 p.m.

   ... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

2. A new platform for fast functional characterization of potentially cancerous mutations

   Type

   Blog Post

   Published

   May 7, 2019, 12:39 p.m.

   ...identify mutations of unknown importance in cancer Previously available methods for investigating and functionally...that that confer proliferation advantages will be revealed in the absence of growth factor. The team assigned...

3. IDP and your PI: A Roadmap for Career Planning and Personal Development

   Type

   Blog Post

   Published

   July 21, 2015, 2:30 p.m.

   ...honest feedback DePace’s yearly planning meetings revolve around two simple worksheets, each of which is ...sections, the trainee speaks first for each section, preventing the meeting from feeling like an assessment or...

4. Easing the Protein Purification Process with pCri

   Type

   Blog Post

   Published

   June 19, 2015, 3:08 p.m.

   ... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...

5. Science as Art

   Type

   Blog Post

   Published

   March 2, 2015, 3:13 p.m.

   ...x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of familiar flowers...such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible with ...

6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

   Type

   Blog Post

   Published

   Dec. 17, 2013, 2:47 p.m.

   ...another is not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor...and more. It is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is...

7. What's New in CRISPR - March 2020

   Type

   Blog Post

   Published

   March 10, 2020, 1:15 p.m.

   ...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...multiplexed gRNAs in human pluripotent stem cells. Previously, CRISPRi or CRISPRa depended on continued expression...

8. What's New in CRISPR - September 2019

   Type

   Blog Post

   Published

   Sept. 10, 2019, 12:56 p.m.

   ...assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity including an ...motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform pooled...

9. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   Published

   Sept. 29, 2015, 2:30 p.m.

   ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding... resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...

10. Mapping the 4D nucleome with CRISPR/Cas9

    Type

    Blog Post

    Published

    Aug. 11, 2015, 2:30 p.m.

    ...transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...