We narrowed to 614 results for: cat.1

... to just that particular assay.      Fig. 1: A native protein (left) is denatured, allowing the...Conformation changes usually fall into one of two categories: ones induced by the researcher and ones that...naturally, by binding to other proteins, changing locations, or even just as part of performing a specific...it a transmembrane protein, or is it know to translocate or fold in certain conditions? It can help to...

...evidence through education.  My only criticisms of what I saw are that we, as a society, need to: 1) use more...impartial. The best science happens when scientists have 1) the courage to make mistakes and 2) the integrity...also a science communicator, UBC Public Scholar, and a member of the Vancouver SciCATs. Follow her on ...researchers and supporters of science marched to advocate for scientist participation in government, evidence...promote help motivate you to spend a little time advocating for science and getting yourself out there for...April because devaluing science has alarming implications for our public policies—many of which impact...scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching experience that...

...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...change offers a significant improvement and simplification of the original technique, specifically in ...

...updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors...

...can also rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution” curve...would be a good time to talk about the amplification and verification processes we use to ensure high-quality...your own experiments. Library amplification 101 Library amplification seems straightforward: E. coli ...transformed with the library DNA, they grow and replicate, and then their DNA is harvested, much like one...library has its own depositor transformation and amplification protocol, but the overall workflow is similar...ll need to remember when undertaking library amplification and sequencing. Maintain representation of the...electrocompetent Stbl4 cells when performing amplifications, and we use electroporation to ensure a good...

...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently...(stay tuned for our affinity purification tag blog!).   Applications of epitope tags   The...success rates across all applications. You can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal...protein purification is not ideal with Myc, as low pH conditions are required during the purification, which...experienced. Finding an antibody that works for your application, specifically detects your protein, is species...and have been widely used across species and applications. In this blog, we will review the most common...that are bound by antibodies for the purpose of purification or detection of the tagged protein. These peptides...

...References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic biology education kit." Science...plasmids and their potential educational uses. We believe that biology education is something that should ... expensive tiny amounts of reagents are, how complicated (and again, expensive) the different machines...desire to create a set of low-cost, easy-to-use educational biology activities – that any school could afford...proteins without the need to handle a living, replicating organism. Furthermore, cell free systems can ...in adapting your research ideas into hands-on educational activities using the BioBits platform, please...BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances 4.8 (2018): eaat5107. PubMed...

...into three main effector functions.  Figure 1: Fc effector functions in an immune response to a ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in...

...the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the passive...at a certain frequency, and this frequency can indicate behavioral state, for example, if someone is awake...

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...

... Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...

...enables the high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier... interference. In the first two months since publication, the lab got over 100 requests for the plasmids...

...materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,...

...difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a) Cells ...  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications and work...ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have the potential ability...point mutations. They allow single-nucleotide modifications to edit DNA without requiring a double-stranded...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-...methods at various genomic loci in four different categories.     Brookhouser, N., et al. BMC Biol 18...MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding sites in a wide range...

...vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein transduction: unrestricted...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may ...that components in the buffer used for protein purification must be helping the untagged protein enter cells...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly...Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR/Cas9 stem cell...

...do researchers track fluorescently labeled cells? 1. Epifluorescence microscopy This technique uses a ...of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...relatively little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence...

...occur, but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...scientific and technical training is that these educational paths prepare you for a vast selection of career...tech transfer, management consulting, science communication, policy and the diverse options in nonprofit...science is much like industry but the “product” is publications—the pressure to produce is the same You get ...and teach students Collaborations may be less “complicated” - for example, easy to initiate a collaboration...is different about non-academia? Scientific publications are a nice to have, not a need to have Must ...every 3-6 years—a short and exciting ride Complicated matrix structures are common Small integrated...

...Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...cassette is removed, the effector is expressed. By truncating one of the recombination sites in the construct...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image ... reporter (blue) and stained with PI (pink) to indicate cell death. Image from Diessl et al., 2020. ...https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining...protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison...

...extension. Now, they split EB1 into two halves (Fig. 1): one containing the microtubule binding domain and...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature...versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9 cleavage activity ...plasmid that allows for rapid fluorescence-based quantification of base editors that utilize dCas9 fused to... The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence is...frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies simply involves tracking...

... contain the full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12... (AmpR) and origins of replication (ori and f1 ori) which allow it to replicate.   How can I prevent...the bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...the recombined plasmid is still visible. This indicates that the DNA prep contains a mixture of the full...