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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: CAL;

Showing: 691 - 720 of 845 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...presence of the CPP may alter protein function or localization. Thus, the usefulness of CPPs must be evaluated...

  2. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring meetings a priority. Schedule meetings or calls regularly and don’t cancel, so that you drive progress...

  3. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...developed a web-based, career-planning platform called the Individual Development Plan (IDP) and uses ... Outreach activities (e.g. Volunteer at a local high school science class: Science Club for Girls...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...arrays, they did not know their functions and simply called the repeated elements “direct repeats” and the ...

  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out. Conclusions...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... the following, which are later printed for my physical lab notebook, along with any figures generated...between each word can make it difficult to accurately call a file. Version control your commonly used scripts...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability... and “Sequence” tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... a downregulation of signal, though it’s hard to call from this plot. The red (bottom) peak overlaps with...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

  14. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...intuitive, or logical to a biologist that are not at all obvious, intuitive, or logical to a layperson...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...once it is engaged by its target, it will non-specifically cut ssDNA. This feature is shared by other Cas... potential as a viral therapeutic that could specifically degrade target viral RNA. For more information...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...out the sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well...answer to these questions--which were real, not hypothetical--was clearly “no.” When there was room for misinterpretation...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...library, propagated it, separated it into roughly identical replicate groups, and treated these groups with...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  18. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...Another strength of Golden Gate cloning is its scalability. Unique 4 base overhangs can be used to assemble...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...

  19. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ... will provide an initial consultation meeting or call for free.  Take advantage of this to get the information...blogged for us) offers a free report on her website called “Secrets to Success in Graduate School”. She has...

  21. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing...

  22. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...characters. A descriptive purpose focuses on the practical application of the plasmid and will help a scientist... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

  23. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...given the data provided by the depositing lab, we call these “quality control (QC) issues.” We then ask..., Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation...

  24. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...to visualize DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

  25. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...search algorithm rather than pre-designed sets, so identical searches can return multiple answers. You might...might want to take advantage of their save and recall functions when you get results you like. NEBridge ...

  26. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... gRNA (Anzalone et al., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., ...other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created with BioRender.com...

  27. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... for PAM-less editing   Guides for CRISPR are typically selected by the ‘quality’ of the gRNA sequence...the Liu lab through phage-assisted evolution, specifically with the goal of generating Cas enzymes with...

  28. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...however, your cost per cloning reaction drops dramatically, as one tube of enzyme should be enough for ... guarantees, some cloning techniques do tip the scales in your favor. For starters, Golden Gate cloning...

  29. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...results out there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols...

  30. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...sequence (gene). In a clean sample, there are typically fewer than 10 hits to a given sequence, and we...control is recommended but not yet required for clinical-grade materials, and is not commonly performed...
Showing: 691 - 720 of 845 results