We narrowed to 600 results for: ELP

1. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ... viral vector service in 2016...we’re excited to help scientists share their reagents with the scientific...

2. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...involved). The new homepage and navigational elements help users who prefer to browse and discover items in...

3. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...advantages and limitations of each Cas enzyme can help determine which enzyme to use. Remember, accessibility...

4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...replication-incompetent phage particles with assistance from a ‘helper’ phage prior to transduction. Scientists also use...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ... homologs. Evolving better base editors with the help of phages! The latest ABE from the Liu lab is ABE7.10...

6. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...materials. The use of reporting guidelines can also help remind us to include all of the essential details...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...of virus with various tropisms.  Packaging using helper-free packaging cell lines This method utilizes ...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ... and disadvantages of monoclonal antibodies will help you make the best decision for your experimental...

9. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ... blogs on virus safety and viral applications to help you start (safely!) thinking about the possibilities...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ..., as compared to wild type, would be extremely helpful as a complementary approach. Also, in principle...

11. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...Thankfully, there are many great resources available to help guide you through the rough waters of mutation validation...

12. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...research, modeling patient mutations using SDM can help determine if a variant is causal for a given disease...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...efficient, what other challenges does this system help to solve? Myers: Only a small percentage of the ...

14. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ...brom-indigo). So far, so good, but how does this help us screen plasmids? Blue-white screening in the ...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...P65-HSF1 plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide...

16. Plasmids 101: Yeast Vectors

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    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...  Yeast selection marker What does the marker help synthesize? Counterselection? (growth-based positive...

17. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...was established as an e-commerce company, which helped it stay ahead of the curve as access to the Internet...

18. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...components of your experiment. These considerations will help you narrow down which antibody format to use for...

19. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...used to ensure specificity; this is particularly helpful when wanting to target specific neuron subtypes...

20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...kilobases, but only in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective ...