We narrowed to 553 results for: ESC
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TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...
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Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein degradation... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....driglar. References 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....down your own results; are they the same as the description the authors give? Ask yourself if the results... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... Combining this with some of the loxP methods described above, a genetic modification can be restricted... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....cardiovascular disease, cholesterol gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m...., can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....partners other than those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....Wen, B., Kraus, C., Miller, N. W., Steger, R. J., Escude Velasco, B., Bosch, J. A., Zirin, J. D., Viswanatha... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then compare the... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...