Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 711 results for: Met

Showing: 461 - 480 of 711 results

  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...

  2. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to...

  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang...

  4. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...sensitive to sunlight and prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012...

  5. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...description of the lab’s iTOP protein-only transduction method and its potential applications to CRISPR/Cas9 genome...osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies showed that...

  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP to track...pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab...

  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane ...Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed...

  10. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...educational uses. We believe that biology education is something that should be available to everyone, and hope...

  11. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...simplifies the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric...emission peak around 600nm?  Simply input these parameters and you’ll find matching FP suggestions. If ...

  12. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

  13. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...visualizing glutamate neurotransmission.” Nature Methods (2013) PubMed PMID: 23314171. PubMed Central PMCID...variants of the glutamate sensor iGluSnFR.” Nature Methods (2018) PubMed PMID: 30377363 Aggarwal, Abhi, et...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” of all of...UTR were translationally silent. Both of these methods increase our understanding of translation dynamics...

  16. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary journals...represent different research areas, target readers, methodologies, or qualities of research our adjustment controls...

  17. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...sequencing have prevented laboratories from using these methods for routine processes like plasmid validation. ... problematic for conventional NGS library prep methods because the yield or quality of individual libraries...

  18. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Sales Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting...Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer...

  19. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...

  20. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...
Showing: 461 - 480 of 711 results