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Showing: 461 - 480 of 699 results
  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” of all of...UTR were translationally silent. Both of these methods increase our understanding of translation dynamics...
  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary journals...represent different research areas, target readers, methodologies, or qualities of research our adjustment controls...
  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...sequencing have prevented laboratories from using these methods for routine processes like plasmid validation. ... problematic for conventional NGS library prep methods because the yield or quality of individual libraries...
  4. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Sales Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting...Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer...
  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...educational uses. We believe that biology education is something that should be available to everyone, and hope...
  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...simplifies the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric...emission peak around 600nm?  Simply input these parameters and you’ll find matching FP suggestions. If ...
  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP to track...pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab...
  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane ...Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed...
  9. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...medicine and more successful experiments. It’s something that all scientists should understand when stepping...
  10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...visualizing glutamate neurotransmission.” Nature Methods (2013) PubMed PMID: 23314171. PubMed Central PMCID...variants of the glutamate sensor iGluSnFR.” Nature Methods (2018) PubMed PMID: 30377363 Aggarwal, Abhi, et...
  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...
  12. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...
  13. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...
  14. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...lab? Obviously, funding and support are crucial parameters, and familiarity with equipment always helps.... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...
  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...
  16. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...One common biosensor class is designed to monitor metal ions. Within these ions, calcium is by far one of...
  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...deliver these systems virally instead of by another method? Viral delivery is attractive due to the ease of...viral vectors instead of a traditional transfection method. This is because viral systems have a high infection...
Showing: 461 - 480 of 699 results