Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 850 results for: NTS

Showing: 461 - 480 of 850 results

  1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...selection strategy for their C. elegans/CRISPR experiments, but this strategy required the use of unhealthy...Other labs have used PCR-based screening to detect mutants, but this method limits screening to a few hundred...hundred worms, making the detection of rare events difficult. Dickinson et al. sought to create a new and.... This novel, rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ...matched it with my CV and added more business-like elements. At this point you may be asking yourself, "Why...

  3. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...When preparing to graduate from university, many students are confronted with the question ‘what now?’ This...sectors such as forensics currently have too many applicants compared to the number of available roles). Make...useful to search online job websites to see what requirements or qualifications are needed for the role, as...you get your foot in the door. Match the job's requirements to the content in your Resume and Cover Letter...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...others to see what you actually found, to providing reagents in an accessible and consistent way so other researchers...methods sections, including clear information about reagents, and why other publishers and organizations have...in their communication or making their data and reagents readily available. What if that could change? ...scientific enterprise will benefit. We’ll have fewer students and postdocs sitting frustratedly in reproducibility...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... to treat human genetic diseases, and several treatments are currently being tested in clinical trials...recombination in cultured intestinal stem cells of patients with cystic fibrosis (8).   The difference with...correction in adult stem cells isolated from two patients with cystic fibrosis. They were able to demonstrate...Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013...

  6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...viewed publicly and stored in one place if the participants used the conference hashtag after the conference...

  7. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... the fluorescent toolbox Modern neuroscience experiments often rely on multiplexing, where multiple fluorescent...green, yellow, and/or red sensors in multicolor experiments. With OCaMP, researchers can image multiple brain...F0 (the measure of signal change during calcium events), Greater brightness, Less signal distortion from...above. The number of action potential (AP) spiking events are indicated below the trace; asterisk indicates...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...in 2018, our Product Team) continues to make improvements to our website and inventory management systems... were waiting. I look forward to sharing new developments on this project and on all the interesting things...

  9. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt... Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts Tips for Improving...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... how can you use these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral...more! Libraries allow you to test thousands of elements simultaneously in your system instead of just ... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have already...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... transgenic lines that can be used for other experiments. And, while the BLITZ system was designed and...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...while potentially allowing for up to 20-plex experiments with one visual barcode per cell and up to 200...

  12. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...activity is crucial for successful CRISPR editing experiments, there was a real need in the scientific community... in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac...activity and sgRNA efficiency as well as enrich for mutants through the presence of a fluorescent reporter.... best sgRNA to ensure success in your CRISPR experiments. But how? There have been several systems to ...

  13. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...Addgenies involved). The new homepage and navigational elements help users who prefer to browse and discover items...implementation, we laid the foundations for many improvements to come. Ultimately, we would like our search... will give our new search engine a try to find reagents and educational material. Let us know what works...

  14. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...to a lacZΔM15-mutant bacterial cell in trans complements the mutation allowing for a functional enzyme...when expressed, is lethal to the cell. Cloning fragments are inserted into an MCS in the center of this... susceptible to or dependent on certain media components, and are rescued by genes supplied on the transformed...Expression Systems Find Empty Backbones for Your Experiments    ...

  15. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can... people. At large conferences, look for smaller events or meet-ups to connect with others more easily....conference Make sure you have a business card – even students and postdocs. There are many inexpensive/free ...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will...plasmids (like your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has...researchers have enough information to replicate your experiments, making research more reproducible and your results...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Scientists around the world have been making major improvements to CRISPR technology since its initial applications..., heterodimerizing domain to each of the Cas9 fragments, the authors created a photoactive Cas9 tool, ...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical...process by aligning institutions with standardized agreements and electronic signatures to dramatically speed...nominated for it, it just reflects how useful the reagents we created are for the community. More than 100...were useful for researchers wanting high quality reagents, and we realized we could use the same principles...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...microbiome to deliver a genetic payload to the recipients using the IncP⍺-family-RP4 conjugation system...they only identified seven genera in in vitro experiments, possibly because in vitro conditions did not...The vector retains the oriT for delivery into recipients by conjugation. The labs screened for the host-range...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...of dsRNA Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by ...bidirectional transcription or as inverted repeats. These elements are almost always epigenetically silenced, but... are important considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that...
Showing: 461 - 480 of 850 results