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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 753 results for: Nes

Showing: 461 - 480 of 753 results

  1. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...online from your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at...to generate the legal agreement (MTA), which streamlines the process and saves you time. Anyone affiliated...

  2. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and...Most chromatogram viewing programs (even the free ones) allow you to edit the sequence.   I hope these...

  3. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian...

  4. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...marker excision method for marking distinct cell clones developed by the Michalis Averof lab. It’s an alternative...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start something... publications like FierceBiotech to “learn the business” Attend events with local networking and trade...

  7. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...

  8. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ...

  10. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...involved with COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…).  How to...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports...

  11. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...future. Over the years, we have benefited from the business rigor and efficiencies typically adopted by for-profit... Chonnettia Jones...

  12. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167...

  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression...

  15. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize...

  16. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making...

  17. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were...

  18. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ...

  19. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door...

  20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Editing Consortium hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing...non-homologous end joining (Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: ...
Showing: 461 - 480 of 753 results