We narrowed to 580 results for: SIM

1. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

2. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error prone PCR, EvolvR uses...

3. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP...

4. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria do the...

5. Plasmids 101: Blue-white Screening

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   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...of this gene, disrupting the lethality. This is similar to α-peptide DNA disruption in the blue-white screen...

6. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...much all investigators. Our kit makes it extremely simple to design TALENs. It only takes two to three days...

7. Antibodies 101: Conjugation

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   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and transcriptome measurement in single...

8. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...outlined in Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time...

9. Plasmids 101: The protein expression toolbox

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   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...

10. Summer SciComm: Preprints

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    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...

11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...example green and red fluorescent proteins) allow simultaneous identification of more than one protein of interest...

12. Human Germline Editing Using CRISPR

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    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...edited embryos proceeded to the blastocyst stage at similar rates to untreated controls. Ma et al. performed...

13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...organisms that are engineered can be shared to simplify the comparison by others. Together, we can build...

14. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...Detecting RNA-protein interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...

16. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. Application information...

17. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...

18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ... and spectral properties, must be optimized simultaneously in order to generate a channelrhodopsin that...

19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...postdocs at other institutions to see if we can similarly convince them to go ahead with raises. What will...

20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ... that actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting ...