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We narrowed to 816 results for: ache

Showing: 461 - 480 of 816 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...HJs are resolved via cleavage in one of two ways - each junction resolution could happen on the crossing...Small edits require as little as 30-50 bases for each homology arm, but keep in mind these numbers may... PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...based affinity purification and mass spectrometry approaches. Recently however, scientists have developed ...miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-..., et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45...future work cost both time and money with estimates reaching in the billions of dollars (Freedman et al 2015... not what most readers want to read. As Susanna Bachle points out in her talk (29:31 - 49:45), there are...

  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...need to check for this in your system and then test each vector before starting your experiments (Rolland...A. (2021). Next Step in Gene Delivery: Modern Approaches and Further Perspectives of AAV Tropism Modification...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...efficiently into dividing immortalized cells lines but reach their limits when it comes to transferring DNA into...been established so far, with safety increasing in each generation. The 1st generation of lentiviral vectors...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... strategies include using engineered ligases to attach a ligand to a peptide tag on your protein of interest...use the same tag for multiple proteins but label each separately before reconstituting, choosing your ...potential benefits and costs before investing in one approach or the other. Finding the right tag for you Protein-based...

  8. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, ...  Rachel Leeson...

  9. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...our speed round data presentation, with two winner each receiving a $500 travel award. All participants ...  Rachel Leeson...

  11. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me... Rachel Leeson...

  12. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene...  Rachel Leeson...

  13. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...our tips in our new series: Addgene Lab Tips! In each short episode of this series, we’ll highlight one...that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves ...

  14. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...'ve all got different areas of expertise, we are each equally excited to talk to you, answer any questions...  Rachel Leeson...

  15. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...good starting points to understand the contours of each topic. The video channels are also underrated and...additional resources that might be helpful, please reach out to me by email.  About the author Niko McCarty...

  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...and SITN The Most Difficult thing Vini, Amy, and Outreach Scientist Jessica Welch Have Had to Explain Importance... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...

  17. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ... acid sequences that got closer and closer to achieving it. Once they designed the protein computationally...drugs, all developed with their protein design approach. Congratulations to David Baker, Demis Hassabis...

  19. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks... Rachel Leeson...

  20. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ask you to share your...different fields. Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your...
Showing: 461 - 480 of 816 results