We narrowed to 533 results for: erf
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TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...
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CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....increase HDR frequency by committing a break to performing HR before other factors were loaded onto the ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore fusions on... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m.... can be stored right away. Every week, the lab performs an average of 140 transformations. The lab typically... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA GEGEIPAPLA... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m.... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m.... in your expression host of choice, you should perform a functional assay to make sure your protein isn... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....you realize. Being at a university provides the perfect platform for practicing initiative and for training... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....specified discrepancies. Any sequences that are a perfect match from this script can then continue in the... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens in bacteria... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m.... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....discussed some of the general considerations for performing behavioral tests, but now we’ll focus on how ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....together with information about the genome are powerful resources for investigating any biological process... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions...