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... citations for recent articles. Of course, a publication in Science or Nature is always nice, but in the... lesser-known journal as it is in a top-tier publication. Accessibility is surely also behind the increase...increase in citations for older publications. (As an interesting aside, Nature recently reported that the...in a solution.) But are citations and journal publications really the best way to measure impact? As that...papers detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific...and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing ...consider and quantify the impact of scientific publications. Ventures like Impact Story seek to find ways...

... and chemical contaminants that have been used during the purification procedure. Good indicators of DNA... is necessary for bacterial transformation of chemically and electrocompetent cells is minimal (picogram...nm should fall ~1.8 - 2 and give scientists an indication of purity versus protein contaminants. The 260...nm ratios should fall ~2 - 2.2, and provide an indication of purity versus chaotropic agents as guanidine...addition to being free of any nucleic acid and chemical contaminants, these DNA samples must also be entirely...

...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and IT10 expressed well...and the chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again...detrimental effects on cell size and growth rate, indicating that they compromised protein-protein interactions...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

...several ways. Below is a table listing some of critical differences (adapted from Zhou and Melton, 2008...this process. Moreover, it is much easier to genetically modify cells during the reprogramming process...This opens up a wide range of possibilities in clinical situations. In cases where the objective is to...Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. She...joined Cell Press to pursue a career in science communication and publishing after completing her postdoctoral...from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ https://...PMCID: PMC2854424. 23. Burridge, P.W., et al., Chemically defined generation of human cardiomyocytes. Nat...

...Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs The CRISPR...activity with a fluorescent protein--no affinity purification required! The team used other molecular techniques...compartments in mammalian cells By Angela Abitua Genetically encoded fluorescent proteins are often used to...collaborators have an ongoing effort to improve genetically encoded fluorescent markers for labeling specific...authors generated and described several improved genetically encoded fluorescent markers tagged with mTurquoise2...beads. Workflow of nanobody expression and purification. Iwaki et al., Protein Expr Purif, 2020. ...dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery. ...

...written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute. When it... an exciting class of compact genome editors, typically only around 400 amino acids in size. To give a...-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome... a single experiment (Karmakar et al., 2024). Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The...The process for cloning the 20-nt guide RNA is identical for both vectors. Here, we provide a step-by-step...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...elaborate protocols are available in our earlier publications (Karmakar et al., 2024; Karmakar et al., 2025...

...Within that research group, you will also typically receive technical mentorship from a ‘bench mentor’. In ...receive after you’ve found your mentor, though. Typically, you will identify a ‘scientific mentor’: someone...scientific project. Your scientific mentor is typically a professor who operates a research group. Within...to do? Walk through the protocol mentally (or physically where relevant) and identify any gaps or questions...bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going to each...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...experiments. Unlike in lab courses, where everything is magically made up by helpful TAs or assistants, and sitting...

... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and named this...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...signal inside the tumors was overlaid with the anatomical B-mode images in greyscale.   ARG expression...of the mARG plamsids into HEK293T cells using a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm...authors: Marjorie Buss is a PhD student studying chemical engineering. Mengtong "Tom" Duan is a PhD student...

...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...from the science of others. Improving science communication through video     Video abstracts about research... to be that video is the future in terms of communicating ideas. In 2012, for example, 3 billion hours...video usurps the written word as the means for communicating research. Short videos of 3 to 5 minutes provide... researchers to leverage the video medium to communicate their research more effectively.” How to share...concentration of citations, 1900–2007." Journal of the American Society for Information Science and Technology...study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene...

... line from a reputable distributor such as the American Type Culture Collection and freeze as many early...contamination. For those labs that prefer a more economical mode of detection, Uphoff et. al. outline a do... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...virus. To circumvent this problem, transductions typically employ the positively charged polycation, polybrene...establishes the best conditions for specific applications, stick with them! Changes in such variables ...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85. Pubmed PMID: ...

...conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution). I... science communication and outreach - if you'd like to learn more about Science communication check out...listening to talks all day. But nope, not at the American Society for Microbiology 2018 Microbe meeting....biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization...Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources on Addgene.org Check...

...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was...for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses on the lungs...Center for Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell...Pathology and Laboratory Medicine. He attends in the Medical Intensive Care Unit, the Pulmonary Consultation...the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem cell...Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our sound engineer/producer...

...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman...mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity of previous versions... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it for tissue ...architecture of a fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods...neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553...

...insights into the complicated physical organization of the brain and other biological systems. Interested...deletions are defined using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions...of farnesylated derivatives enables labeling of delicate axonal and dendritic processes not previously ...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving...temporal control without the need for germline modification, and enables Brainbow to be used in a variety...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...

...content of this article. Biosensors (‘biological sensors’) are biological tools that monitor a process or detect...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent..., and you should always check the associated publication for the specifics of your chosen plasmid. Types...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated... Jessica Welch...

...attended both online and on site courses with the American Society for Microbiology (ASM) Scientific Writing...of any scientist’s career. We use writing to communicate our findings and share them with the world. If...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...sessions and reinforced our newfound knowledge with practical understanding of how to apply it. The personal...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

... some of the drawbacks. Specifically, viruses are able to infect and replicate in host cells, but they...gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site.   The...Anderson Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...Foundation study showed that 57% of PhDs in US Biomedical workforce will NOT go into “traditional” academic...tech transfer, management consulting, science communication, policy and the diverse options in nonprofit...science is much like industry but the “product” is publications—the pressure to produce is the same You get ...and teach students Collaborations may be less “complicated” - for example, easy to initiate a collaboration...is different about non-academia? Scientific publications are a nice to have, not a need to have Must ...every 3-6 years—a short and exciting ride Complicated matrix structures are common Small integrated...

...protein is (localization) and how much there is (quantification). But what if you’ve got several interesting...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping out...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...yourself stuck with red-green images generated automatically from the microscope software, you can always...it, the secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

...Musicophilia includes case studies of relevant neurological atypicalities, including a patient with amusia - the...capture the attention of even the most skeptical reader of historical fiction (I can personally attest to...sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the...times."     - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading...