Skip to main content
Addgene
Showing: 461 - 480 of 669 results

  1. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a... a higher titer due to the smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid...

  3. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it’s clear that the...Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  6. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...PCR Catch up on Your Plasmid Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty...Additional Protocols Browse Our Special Collections for Plasmids from Your Field ...

  8. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...ability to use an RNA guide to target other nucleic acids—usually DNA—to cut, nick, or bind. DNA CRISPR array...record of previously encountered foreign nucleic acids. The unusual sequence of these arrays, with alternating...

  9. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...methodologies and rigorous research plans. However, amidst the organized chaos of lab research and PhD studies...maintaining an openness and looseness of spirit. Amidst the rigors of PhD studies and long days in the ...

  10. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...quality candidates are selected.      CRISPR aids in multiple stages of the drug discovery pipeline...resources on Addgene.org CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development ...

  11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org Check...Reference Find protocols on plasmid cloning Find plasmids for your research ...

  12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...

  19. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate... a virus, you need only transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral...
Showing: 461 - 480 of 669 results