We narrowed to 486 results for: mme

1. Antibodies 101: The Basics of Western Blotting

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   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

2. CRISPR 101: Off-Target Effects

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   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...localizes to unintended sites and performs its programmed function there, this is an off-target effect....

3. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

4. Plasmids 101: Repressible Promoters

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   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

5. Sequencing Options for CRISPR Genotyping

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   Blog Post

   Published

   Oct. 4, 2016, 2:30 p.m.

   ...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology...

6. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

7. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

8. Finding Your Science Policy Path

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   Blog Post

   Published

   Sept. 22, 2020, 1:15 p.m.

   ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

9. A History of Genome Engineering in Popular Culture

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   Blog Post

   Published

   Feb. 25, 2020, 2:15 p.m.

   ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...

10. Quick Guide to All Things Lentivirus

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    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

12. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

13. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

14. Typing CRISPR Systems

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    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

15. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...   Figure 1: A chart with MoClo kit recommendations for different modal organisms and applications...

16. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ... glass and the water-like buffer the sample is immersed in. Although the excitation light is completely...

17. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ... effector function. Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic...

18. Antibodies 101: Flow Cytometry

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    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...arising from the secondary antibody.  It is also recommended that users include a viability control as this...

19. Antibodies 101: Introduction to Antibodies

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    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ... antibodies for neuroscience research from the Trimmer lab ...

20. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...viability. Avoid Low Quality/High Quantity – We recommend avoiding a High Quantity/Low Quality mindset for...