We narrowed to 507 results for: otos

1. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...hours reviewing papers to piece together the best protocol;  he wanted to get going and learn through doing...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

5. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...Overview: Generation of Gene Knockout Mice". Curr Protoc Cell Biol. 19: 1217. PubMed PMID: 19731224 PubMed...

6. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ...a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in...

7. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...

8. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),...

9. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...

10. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ... the bench, this results in a shorter staining protocol because the primary antibody can be “labeled” ...

11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...-use AAV from Addgene here or check out these protocols if you want to package your own virus. ...

12. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ... Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1. Anderson JC, Dueber JE, Leguia ...

13. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

14. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...later years, improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H...

15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...transformation, preparation, and analysis from our protocols and molecular biology reference pages! Resources...

16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...including a lot of future Addgenies as shown in the photo below.  Kids and adults alike enjoyed some friendly...

17. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ... function. More detailed information and some protocols can be found in the references provided. Additional...

18. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...these techniques to optimize our lab space, our protocols and even our Lab Inventory Management system. ...

19. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...in E.coli as simple as following a DNA miniprep protocol!  First, the authors created the pMAK backbone...

20. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...