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We narrowed to 599 results for: REV;

Showing: 241 - 250 of 599 results

  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial rather...toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila, so...

  2. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...to control their own future in science (see my previous blog on this).  Indeed, that was a primary message...rules and practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled...

  3. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...variety of careers outside of academia. She has had previous graduate students and postdocs go on to work in...what you are truly passionate about. Check out my previous post if you're looking for more information about...

  4. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral infection into target cells that have previously been stably transfected for Cas9 expression. ... in bacterial toxin resistance, identifying 4 previously unknown genes.  “With this work, it is now possible...

  5. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...bacterial chromosome that stores a genetic record of previously encountered foreign nucleic acids. The unusual...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/...

  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...the Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans...Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...and the species they are compatible with are all reviewed here. What are affinity tags? Affinity tags are...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages...

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...organism, all cells of that type are affected. Previous tools to overcome this restraint include an AAV-based...into an attB site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites...
Showing: 241 - 250 of 599 results