We narrowed to 575 results for: BASE;
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TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...
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Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....populations. Figure 2: Compensation example based on experimental sample. Flow plots showing a sample... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid. The Addgene Vector Database has more information about common vector backbones... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....Hopefully we will have new tools for plant science based around those technologies in the near future. Science... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....CREATE method? CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....together a really great solution that covered all the bases. You may have also used your writing skills to convey... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...