We narrowed to 556 results for: ESC;

1. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...

2. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...Wen, B., Kraus, C., Miller, N. W., Steger, R. J., Escude Velasco, B., Bosch, J. A., Zirin, J. D., Viswanatha...

3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...the repertoire of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version...

4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available...

5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the...

6. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

7. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws...

9. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...ability to complete the project on such a short timescale. Our article ultimately reported the development...

10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

11. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...

12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

13. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

14. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ... assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from entire...

15. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...

17. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

18. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ...

20. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...