We narrowed to 872 results for: FER

...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...

....  3. Do you prefer being a generalist? Working on a variety of projects and in different fields of research...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...

...interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional approach of an autonomously...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these...YGOI on a western blot or under a microscope, we offer a collection of vectors carrying several fusion ..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

...plasticware or think about conserving reagents and buffers.  However, I had very environmentally conscious...required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying..., I was bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took... small enough to minimize the required area and buffer volume.  So, I designed my own gel box (see illustration...

... together as useful and productive, forming a different constellation of interests and skills than what... experimental design and data with authors and referees. And I am constantly teaching – often explaining...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...short program to build skills and connections. AAAS offers a summer program that gives graduate students an...

...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get ...

...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound...40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...

...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...

...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...for and in HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight...

...outside of the cell. The same protein may have different epitopes available if it is being selected out...controls in validation, as you may need to test different configurations and conditions to understand exactly... what a given protein is doing or changing in different conditions. Just remember to keep good records...when you’re building your antibody library! References and Resources More resources on Addgene’s blog...

...National Institutes of Health.     References and Resources References Bradbury A, Plückthun A (2015) Reproducibility...). Recombinant antibodies are plasmid-based and offer numerous advantages, including more secure archiving..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

...paper is much more detailed. I prefer to do it on another day - preferably the following morning - as usually... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...

...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...

...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes...viability question. The miLab you see today is far different than what was originally planned, due in large...the potential contacts your personal network can offer, you are really on your own to build a strategic...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible...

...specificity of their luciferase assay, they concluded that components in the buffer used for protein purification...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...

...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...

...side of caution when adding buffer back to the solution. Add a bit less buffer than you would to achieve...process of generating viral particles. Limit tube transfer - the fewer opportunities your viral particles...working on methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant...

...Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership was fruitful...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...

...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...and temperature and leaf autofluorescence can interfere with microscopy. Thus, Bernach sought to develop... Jennifer Tsang...

...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature...