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We narrowed to 867 results for: FER

Showing: 481 - 500 of 867 results

  1. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...for and in HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight...

  2. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... together as useful and productive, forming a different constellation of interests and skills than what... experimental design and data with authors and referees. And I am constantly teaching – often explaining...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...short program to build skills and connections. AAAS offers a summer program that gives graduate students an...

  4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...paper is much more detailed. I prefer to do it on another day - preferably the following morning - as usually... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...

  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...outside of the cell. The same protein may have different epitopes available if it is being selected out...controls in validation, as you may need to test different configurations and conditions to understand exactly... what a given protein is doing or changing in different conditions. Just remember to keep good records...when you’re building your antibody library! References and Resources More resources on Addgene’s blog...

  6. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...National Institutes of Health.     References and Resources References Bradbury A, Plückthun A (2015) Reproducibility...). Recombinant antibodies are plasmid-based and offer numerous advantages, including more secure archiving..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P...

  7. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get ...

  8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound...40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...

  9. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes...viability question. The miLab you see today is far different than what was originally planned, due in large...the potential contacts your personal network can offer, you are really on your own to build a strategic...you believe your idea has potential to make a difference, then make sure you’re able to answer every possible...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...specificity of their luciferase assay, they concluded that components in the buffer used for protein purification...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...

  11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...Sequencing and QC at Addgene.  References and resources References Mosch, R., and Guchelaar, H.-J. (... T-cells or monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through...vitro approaches enables researchers to develop safer and more effective mAb therapeutics. The in silico...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...side of caution when adding buffer back to the solution. Add a bit less buffer than you would to achieve...process of generating viral particles. Limit tube transfer - the fewer opportunities your viral particles...working on methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant...

  13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...and temperature and leaf autofluorescence can interfere with microscopy. Thus, Bernach sought to develop... Jennifer Tsang...

  14. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...

  15. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature...

  16. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership was fruitful...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...

  17. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...tend to be more "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing...presents regularly with labs around the world. You may prefer a more competitive style to spur you to succeed..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

  18. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even...

  20. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...consumer-grade components. In this field, often referred to as 'frugal science', existing components are...problem-solving platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper...microscope. Lower left panel shows an image of differentiating monocyte with planes and hardware to it. Right...image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling...
Showing: 481 - 500 of 867 results