...specificity of their luciferase assay, they concluded that components in the buffer used for protein purification...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...

...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature...

...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...and temperature and leaf autofluorescence can interfere with microscopy. Thus, Bernach sought to develop... Jennifer Tsang...

...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...

...side of caution when adding buffer back to the solution. Add a bit less buffer than you would to achieve...process of generating viral particles. Limit tube transfer - the fewer opportunities your viral particles...working on methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant...

...Scotland, who had just the right infrastructure and offered to collaborate with them. The partnership was fruitful...principal investigators. They also come from many different scientific fields, from social sciences to biology.... We now want to use this potential to make a difference not just against COVID-19, but also in other ...blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...

...Sequencing and QC at Addgene.  References and resources References Mosch, R., and Guchelaar, H.-J. (... T-cells or monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through...vitro approaches enables researchers to develop safer and more effective mAb therapeutics. The in silico...

...tend to be more "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing...presents regularly with labs around the world. You may prefer a more competitive style to spur you to succeed..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

...help define the different cells present in a mixed population, or identify differentiation and disease states...challenges such as the inhibition of immune cell differentiation. Furthermore, TALEN and ZFN require custom ... any immune lineage without affecting cell differentiation in a high-throughput manner. They designed ...

...found in genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular...signaling pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are...cancer mutant collection and the genetic pathway reference collections are described in this paper Systematic...Central PMCID: PMC5386591.  The genetic pathway reference collection is described in this publication ...

...Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that...giving a public talk, ask a friend who works in a different field to listen to you practice.  Ask them what...addition to this annual event, graduate students in different regional and disciplinary communities gather at...here: https://comscicon.com/. All our events are offered free of charge to accepted students, and we pay...

..., without the FMO, it would be difficult to differentiate the Ly6C negative population from the Ly6C positive...like for a particular marker, you can use the reference plots provided by the antibody companies or check... use the APC IgG2c isotype control). The only difference is that the isotype antibody will not target ... as to when you need isotype controls. Our lab prefers to use them to gate our populations, but others...

...Which is why, at the Society for Neuroscience conference in November 2023, we hosted our very first professional... who is my audience, and what do I want to be different for them when I’m done? What are we trying to ...    Here, this image is being used to convey the difference between a gradient tube before and after ultracentrifugation... For instance, you would present information differently in a poster compared to an online abstract or...

...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even...

...consumer-grade components. In this field, often referred to as 'frugal science', existing components are...problem-solving platforms.     Fig. 3: Different workflows involved in sharing open hardware. Upper...microscope. Lower left panel shows an image of differentiating monocyte with planes and hardware to it. Right...image processing and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling...

...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM...Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...

...widely utilized across many different cell types and each one requires different elements for vector propogation... features of yeast vectors and explain how they differ from the bacterial elements previously described...auxotrophic markers and includes the associated references.  Yeast selection marker What does the marker...

...stem cells edited to carry mutant versions of 15 different disease-associated genes. Addgene spoke to Musunuru...mutations. Once that's done in stem cells, we can differentiate those mutant and wild type stem cells into any...all have the mutations but they will also have different genetic backgrounds. It becomes more of an apples-to-oranges...suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system...

... microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... Jennifer Tsang...

...final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...ultracentrifuge.  Figure 1: An outline of the different steps in RNA extraction. Researchers at the... Reagent®, RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... ensure DNA contamination is not an issue.  References                 Allewell NM, Sama A (1974) The...