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We narrowed to 839 results for: LEA

Showing: 481 - 500 of 839 results

  1. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI, it’s easy to miss...Starting with a recap of accomplishments, from learning new techniques to finally figuring out a tough...the yearly planning meeting is that the trainee leads the conversation. As the conversation proceeds through...

  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...and easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...on these and other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli...purification. For a more thorough introduction to pCri, please see the original publication cited below. pCri ...

  3. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...some Addgenies. As the gala came to an end, it was clear that the Addgenies had no intention of ending the...tough but memorable one. At the gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

  7. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...materials from the Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids...AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering process will look slightly... the identity of the packaged DNA and serotype. Please note that Addgene cannot perform functional or ...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...the IBEX Knowledge-Base is named on the Zenodo release in keeping with their value of shared ownership...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, ...dCas9 gets a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has ...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

  10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...those collaborations over the years, he and his colleagues built a Molecular Expressions collection including...much easier for scientists everywhere to get a clearer picture of that intracellular world, even as the...promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson...

  11. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...

  13. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...The guides are optimized to maximize on-target cleavage and minimize off-target effects.   New from ...

  15. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

  16. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ... you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

  17. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...Product Development Manager at eScience Labs. To learn how Caitlin got into scicomm and landed her first...series! Additional Resources on the Addgene Blog Learn more about alternative science careers here Interested... for scientists here Resources on Addgene.org Learn more about careers at Addgene ...

  18. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...require the use of specific sequences. Read on to learn how easy this process is and to get tips for applying...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When ...

  19. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...asking what approach will I take and what can I learn, learning the difference between good and bad data, and...establishes your professional brand. It offers colleagues insight into your interests and will determine...

  20. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...suggesting that outcomes are already determined by at least day 13. However, distinct transcriptional states...335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study ...
Showing: 481 - 500 of 839 results