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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 481 - 500 of 711 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...

  2. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...

  3. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...lab? Obviously, funding and support are crucial parameters, and familiarity with equipment always helps.... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...

  5. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated...January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...

  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...cell (National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies, 1999). This...jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. Monoclonal ...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...One common biosensor class is designed to monitor metal ions. Within these ions, calcium is by far one of...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...deliver these systems virally instead of by another method? Viral delivery is attractive due to the ease of...viral vectors instead of a traditional transfection method. This is because viral systems have a high infection...

  11. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...experts, watch webinars, or use other research methods. In the case of the antibody guide, primary research... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...

  12. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...with other scientists who maybe hadn’t used this method. I enjoyed writing the first post so I signed up... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...

  13. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used...a lot of space and don’t tell you why you did something or what the result was, so try not to do this....

  14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ... from a polycistronic transcript. Although this method shortens construct size, it requires coexpression... be processed, a distinct advantage over other methods that favor certain nucleotides at the 5’ end. ...

  15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV....a large intervening deletion. Not only can this method precisely program large genomic deletions of up...

  17. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

  19. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...before it was cool or a necessary result of the #MeToo movement. We are carefully attentive to genuine ...

  20. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks."Molecular...
Showing: 481 - 500 of 711 results