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We narrowed to 850 results for: NTS

Showing: 481 - 500 of 850 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...approach or indirect approach, depending on the requirements of the specific assay. For the latter three,...indicated when the protein of interest is found in low amounts or if the conjugated signaling molecule has a weak...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...primary antibodies. The introduction of antibody fragments, such as nanobodies, has introduced new options...

  2. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...of dsRNA Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by ...bidirectional transcription or as inverted repeats. These elements are almost always epigenetically silenced, but... are important considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ... Each sequencing service will have different requirements for volume and concentration, and not all providers..., you can use DNA-editing software to perform alignments of multiple sequences to visually check for homology...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

  4. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... third phase of funding, the NURSA Consortium represents a unique funding model in nuclear receptor research...and acquiring them in a timely manner. Addgene represents an excellent solution to the problem of sharing...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... survive in competitive environments — like by living where no one else wants to. Some organisms manage...their host organisms to thrive in competitive environments. The genes encoding these functions form the...cryptic plasmid is among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16....

  6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic promoters with...the retina, it was more likely to have borrowed elements from a natural promoter that was active in the...labeling would be useful for neuronal tracing experiments where lower levels of labeling makes it easier...

  7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust... distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs...TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity while also... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ..., or universities may have someone senior from accounts or HR on the judging panel. It’s important for...Skills 5. Practice makes perfect, apply for small grants too There are actually quite a lot of different... researchers, so look into applying for smaller grants from within your institute or a private funding...the experience I gained in writing small travel grants to attend conferences and a seed funding grant ...

  9. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...companies and organizations selling biological reagents have a ton of data on the catalog page. It’s not.... Although many may not realize it, all of the reagents available at Addgene come from researchers, and...don’t have the resources to recapitulate the experiments performed by our thousands of depositors. That...Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations. Streamline...

  10. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...microbiology meeting. Create an outline: Write key bullet points for what you would like to say to help build your...result, and the fundamental takeaway. Writing these points will help you remember what to say and provide ...outline you created for yourself. Hit the key bullet points and elaborate if your audience seems captivated... the project as a whole. In addition to time constraints, there may be particular poster you are interested...

  11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... corrective amounts of protein for any such disease. This technology therefore represents a potential ...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...produced in multiple different cell types, including plants, mammals, and insects. In this post, we will compare...producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus, so this is...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any... important to you — virus type, cost, time, or reagents and equipment. Hopefully this post has provided...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...number of cycles.  Choosing a method  As with all experiments, a number of factors will determine which approach...Resources needed Most molecular biology labs and departments will have qPCR machines available for use. ddPCR...Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc... The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... it can amplify DNA using only a short list of reagents and several heating and cooling steps. PCR relies...strand of DNA to add nucleotides to.  Using these reagents and a series of heating (denaturing) and cooling...cycle. However, it is common for dNTPs and other reagents to run low during the final cycles which would...of different molecular biology techniques and experiments. End point PCR’s goal is to amplify a specific...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...post-transfection to help remove toxic transfection reagents and keep the cells happy. Harvesting virus Once...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14...sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7. https://...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the degron domain is terminal. Between the tag elements (protease and degron) and the protein of interest...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...

  17. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing... process can be a bit of a mystery to graduate students in academia. Getting a drug to the market usually...pages. In academia, however, writing papers and grants is an inescapable and essential part of being able...’re often required to at least attempt to write grants. If you don’t enjoy or excel at writing, an industry...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...particularly related to the neurosciences.  These developments in microscopy coupled with techniques that make...view of a cleared rat pons, medulla and the first segments of the spinal cord showing the cortico spinal ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human...anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy Modern...

  19. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...require a standard or a reference, meaning saving on reagents (and time)! AAV titering using droplet digital...emulsion technology. Each droplet contains the ingredients for amplification of the target DNA. This partitioning...prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for...digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...is the first of what I, scientists, doctors and patients hope are many more clinical successes for gene...the human condition. Luck brought all these key elements together at the right time and place. But it is...limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE cells using ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...
Showing: 481 - 500 of 850 results