We narrowed to 517 results for: PROC
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TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription, the promoter...
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Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....normalization is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m.... air-water interface (AWI) during the freezing process, resulting in sample loss. Ci Ji Lim’s lab found... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....identify two functionally distinct populations." Proceedings of the National Academy of Sciences 109.2 (2012... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m.... polylinker sequences of molecular vectors". Proceedings of the Belarusian State University Series of ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion The... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....enables labeling of delicate axonal and dendritic processes not previously visible with Brainbow-1 and -2.... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid: the Escherichia... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....You only successfully generate 1 null clone and proceed with it. In this case, the likelihood of having... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....considerably, then the high degree of variability in the process could make it difficult to obtain meaningful results... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m.... and targeting the epigenome may make certain processes, like cellular reprogramming of iPSCs, more efficient... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m.... intimidating use of discipline or competence procedures Threats to personal standing – including undermining... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....Here, NLuc internally excites the fluor through a process known as bioluminescent resonance energy transfer... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....used to better understand and engineer cellular processes that are influenced by temporally ordered biochemical... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....new professional capacity generated during this process result directly in positive impacts on the lives... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....to study gene regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and...