We narrowed to 599 results for: REV;
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TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m...., you’ll be able to use or build on work done previously, borrow small aliquots of restriction enzymes...
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CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....their own unique features and quirks. Here, we will review the genome engineering abilities of the lesser-... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....increase stability. Nature has evolved ways to prevent degradation of important cellular RNA molecules... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m.... - the tech transfer community. In a recent law review article we received some entirely unsolicited praise... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....the proteasome to destroy targeted proteins. We previously developed small molecule degraders that achieve... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....To create their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability.... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....or TE buffer, store them in a -80°C freezer to prevent RNA degradation. They will degrade in a -20°C freezer... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....expression and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....germline editing is permissible in cases of disease prevention. However, it is important to consider that already-tested... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....can narrow them down in a variety of ways. First, review the citations. An antibody that has been cited ... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....the antibody binds to a protein, and then voila! Wherever your protein of interest is, a signal will also... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....strategy was. If you didn’t quite fix everything or revealed another issue in the process, you might need to... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m.... PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase (RT), which induces two double-strand... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....(PV)-expressing neurons, neurons that the lab previously found had a role in perceiving touch inputs. ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations. Finally, if you... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....fragments and you will remove the buffers used in the previous reactions. This will increase your chances to ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....for this purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....formation of the desired ligation product is irreversible because this construct does not retain the enzyme...