We narrowed to 580 results for: SIM

1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...biotechnology today are things like butanol - very simple molecules - a few carbons and oxygens. Nothing ...

3. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase...

4. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...

5. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...

7. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...your virus experiments. References 1. Anthony, Simon J., et al. "A strategy to estimate unknown viral...

8. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...

9. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...

10. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing with...

11. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...these colors so that your text is easily read. Similarly, be cognizant of audience members that are color-blind...

12. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...

13. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...

14. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ... of the transcribed ncRNA (Jiang et al. 2014).  Simple knock-out experiments are trickier when it comes...

16. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...first round of detection are actually gone. First, simply repeat the imaging step to check if any secondary...

17. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...

18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...long hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they ...

19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...after receiving her degree she was faced with a similar conundrum – she had the skills, training, and motivation...

20. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies...