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...or C-terminal fusion based on your experimental needs. Equally important, before cloning you must consider...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of... to use c-fos expression to get the evidence you need. For example, as shown in this study [3], hippocampal...

...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...

... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

...In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded and therefore...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

...their biotinylation. The advantage of APEX is its speed, labeling neighboring proteins in minutes rather...developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous biotin ligases...

...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

...between cells can rescue aerobic respiration. Proceedings of the National Academy of Sciences USA, 103(... system (Figure 6). This approach eliminates the need for expensive reagents and equipment like incubators...

... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

...and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy...induces reanimation from general anesthesia." Proceedings of the National Academy of Sciences 113.45 (2016...

...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...

...efficiency isn’t everything — prime editing typically needs to be optimized for each application, and the efficacy...efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits...

...fast protocol, with only 5 hours incubation time needed for a single round of editing, and a subsequent...another species of Enterobacteriaceae, without the need for modification. This finding suggests that the...

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...That will warm up the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions...