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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 699 results for: eed

Showing: 481 - 500 of 699 results

  1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

  9. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...

  12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...efficiency isn’t everything — prime editing typically needs to be optimized for each application, and the efficacy...efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our...

  15. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...post, we talked about Addgene’s data processing needs, our criteria for selecting a new workflow management...

  16. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ... participants will be invited to compete in our speed round data presentation, with two winner each receiving...

  18. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...through our newly revamped Data Hub! The community needs more data-sharing heroes like you.)  ...
Showing: 481 - 500 of 699 results