We narrowed to 533 results for: erf

1. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...specified discrepancies. Any sequences that are a perfect match from this script can then continue in the...

2. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community...

3. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

4. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   Published

   May 21, 2024, 1:30 p.m.

   ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

5. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...enough information there for you to be independent performing the technique you trained to do? Walk through...

6. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

8. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

9. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ... CM, Partridge SR, Iredell JR (2017) Plasmid interference for curing antibiotic resistance plasmids in...

10. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

11. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

12. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

13. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

14. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

15. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

16. The Challenges of Cell Culture

    Type

    Blog Post

    Published

    May 5, 2016, 2:30 p.m.

    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

17. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

18. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

19. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

20. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...