We narrowed to 679 results for: ids
-
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...
-
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....to 100°C. The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate... a virus, you need only transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....production. Whether redesigning viral packaging plasmids, investigating different viral production strategies... exciting to test this approach with different capsids. Most importantly, because our AAV yields are improved... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....Techniques Catch Up on All Things Plasmid with Our Plasmids 101 Series Get Tips on Verifying Your Plasmid ...Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m.... cellular molecules such as proteins or nucleic acids. This conjunction of vectors and exosome was given...cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction properties.... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...Speeds Science Resources at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....the single and triple abbreviations of the amino acids memorized, but the structures are another story....peptide for some reason. It contains all twenty amino acids plus selenocysteine and pyrrolysine. I have found... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here! ... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....button_text" is_json="true" %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute...on addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged ... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....Engineered 677 61 AkaLumine ATP No Common uses for plasmids expressing luciferase Luciferase reporter assays... plasmids 101... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....Visit our CRISPR plasmids and resources page Read our CRISPR guide Find CRISPR plasmids for mammalian systems... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....genetically encoded, and luckily, we have pre-designed plasmids for these systems! Ion sensors One common biosensor... vehicle, we’ve got you covered with packaging plasmids! References and Resources References Bhalla ... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....recommend you keep an eye out for emerging new capsids in this area. Q8: On dense labeling in the brain...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...editors for your research Read our CRISPR guide Find plasmids for your research ...