We narrowed to 779 results for: ina
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TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m....It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...or lab manager’s approval before letting your imagination run loose on the lab. Have fun! Here’s an example...
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CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert adenosine to inosine. Activate/Repress... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This is useful for both in vitro and in vivo...serotypes pAAV-CAG-tTA AAV2 Molecular Tools Gradinaru New viral service pAAV_hSyn1_nLightG AAV9 Biosensors... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog PostPublishedNov. 8, 2016, 3:30 p.m....we wouldn’t necessarily have expected when we originally sent out the call for applications. Cervical ...caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....you would like us to feature in the coming year. Finally, we are always looking for guest bloggers who want...and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog PostPublishedFeb. 13, 2014, 1 p.m....insert. Example, if you have a Flag tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene...pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....otherwise be lost amid lab closures or project terminations, ensuring that scientific progress made through... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m.... the launch of our fast-growing ready-to-use recombinant antibodies collection, currently composed of ...the community to drive new science experiments. Finally, we were thrilled by how many people accessed our... -
Your Top Requested Plasmid in 2015!
TypeBlog PostPublishedDec. 30, 2015, 3:30 p.m....2015 was... lentiCRISPR V2 LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...cells, deciphering the mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....adding plasmid) to make sure you don’t have contamination. Also conduct a positive control (test a ... Melina Fan... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog PostPublishedMarch 17, 2021, 2:40 p.m....forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity, which raises...Giving think tank event where we brought a multidisciplinary group of scientists together to draft a research... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...adding Cas14 to the mix, you get the ultimate combination enabling the detection of RNA, dsDNA, and now... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m.... timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives...The afternoon program mixed things up with a combination of shorter, more narrowly focused lectures, interactive... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....of the high fidelity version (despite what the original paper says). In their hands, BsaI-HF has produced...they hope others will do the same. “This isn’t the final version,” she said. “If we learn how to make the... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m...., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and... over-expressed, constitutively activated or inactivated the pathway. “The idea is that if you introduce... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....different cell types, at different times, and in combination with different genes. Having CRISPR systems for...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....that you confirm the details and final experimental outcome in the original publication. As you develop and... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....or in vivo testing. We recommend you check the original paper of your AAV plasmid of choice for more information...be available through our distributor partners in China, Brazil, Japan, Korea, Mexico, and India. Once the... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m.... more broadly to accelerate COVID-19 research: Sinai Health System ...Essen University of North Carolina at Chapel Hill University of Illinois Chicago ...