We narrowed to 662 results for: mpo

1. New CRISPR Web Resources and #12DaysofCRISPR Recap

   Type

   Blog Post

   Published

   Dec. 12, 2017, 3:13 p.m.

   ...changes to the genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to...

2. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...preventing the activity of DNase. While this is very important to preserve the stability of your DNA, it will...

3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   Published

   May 21, 2020, 1:15 p.m.

   ...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...

4. Fostering communication in remote collaborations

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   Blog Post

   Published

   April 21, 2020, 1:14 p.m.

   ...scientists working in different time zones, it’s important that everyone can access data and drafts of manuscripts...

5. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

   Type

   Blog Post

   Published

   Jan. 21, 2020, 2:30 p.m.

   ...screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured by BioGRID...

6. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

   Type

   Blog Post

   Published

   June 19, 2018, 1:38 p.m.

   ... et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial viability...

7. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

   Published

   June 5, 2017, 3:19 p.m.

   ...believes there is strength in diversity and in empowering team members to take initiative and give their...

9. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...

10. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...overhangs, insert and vector purification, and, most importantly, excessive time requirements. FastCloning is ...

11. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    Published

    Aug. 19, 2014, 3:39 p.m.

    ...consider as you make your choice. The first and most important thing you need to know is your expression system...

12. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...

13. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    Published

    Nov. 25, 2013, 10:12 p.m.

    ...biological and translative technologies. Addgene has empowered researchers to harness previous experimental successes...

14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    Published

    Sept. 21, 2023, 1:15 p.m.

    ... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

15. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...destabilization can be brought about by the tag so it’s important to validate tagged protein functionality (Arribere...

16. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...learn more. What is an isotype? Antibodies are composed of light chains and heavy chains made up of variable...

17. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...gene expression, growth rates, etc.). Thus, it’s important to maintain short culture periods (less than 20...

18. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...

19. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

20. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust...