We narrowed to 507 results for: otos

1. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...Find ready-to-use AAV from Addgene or check out protocols for packaging your own virus ...

2. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ... streamline your plasmid extractions. Use this protocol shared by Dr. Rob Hurt for high-throughput minipreps...

3. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...nervous system research; however, microglia are notoriously resistant to viral transduction. Through in vivo...

4. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease...

5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...

6. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...Social Networking for Scientists Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments...

7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...fluorescent fusion proteins in cell biology."Current Protocols in Cell Biology (2005): 21-4. Pubmed PMID: 18228466...

8. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse ...

9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...need share data with them including reports and protocols. To facilitate this, some ELNs allow you to invite...

10. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design Tools Read other CRISPR blog ...

11. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...Conventional cryogenic electron microscopy (cryo-EM) protocols typically require high concentration and purity...

12. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...non-surface markers, a separate intracellular labeling protocol is necessary, requiring chemical treatment of ...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ... editing in stem cells using CORRECT." Nature protocols 12.2 (2017): 329-354. PubMed PMID: 28102837. Additional...

14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ... sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end or paired-end...

15. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ... find it. Experienced speakers present useful protocols, geeky science details, products that resulted...

16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...

17. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... if your research is dependent on a pathology microtome/cryostat, then making a careful decision on the...

18. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ... Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene blog:  Plasmids ...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8 Skeletal muscle AAV1...

20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...knockout mouse models using long ssDNA donors.” Nat Protoc. 13 (2018) (1):195-215. DOI: 10.1038/nprot.2017.153...