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  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...fluorescent protein induced by blue light." Nature biotechnology 24.4 (2006): 461-465. PubMed PMID:16550175. ... dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040. PubMed PMID:18724362...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red and Cas9, which...for highly specific genome editing.”  Nature Biotechnology 32(6) (2014): 569-576. PubMed PMID: 24770325...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...have been making major improvements to CRISPR technology since its initial applications for genome engineering...CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...membrane, you can dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins...the bubbles in the membrane sandwich Ensure methanol concentration in transfer buffer is < 20% ...

  10. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...working in fly research, I hope you find this new technology as helpful as we have!  References and Resources...
Showing: 251 - 260 of 460 results