We narrowed to 855 results for: NTS

...connection between molecular- and cellular-level events and behavior. It’s a problem that he considers ...change has a particular advantage for observing events taking place inside cells that are on the move,...Methods, the FRET changes of these Twitch sensor variants were optimized by testing various linker configurations...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat... than intensity-based readouts.” Griesbeck also points out that the approach he and his colleagues took...“trick” to the development of additional color variants. This resulted in the construction of redCALWY...redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor ...

...Together, these limitations create challenges for measurements needed to move discoveries from the lab bench...compatible with both intra- and extracellular environments, making it effective for analyzing real-time...biology. They first identified that in a set of patients with gastric cancer (GC), TP53 was one of the ...mutated genes. Next, the authors explored how these variants of p53 shape chemosensitivity and mechanistic ...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the...which was slowed by knockdown of UBE3C or other components of the degradation pathway. HiBiT enabled the...through extracellular-specific and full lytic measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time...

...long-lasting way to identify and reference documents, files, or physical reagents. What are persistent identifiers... there is insufficient information about which reagents were used? Unambiguous identification of a reagent...Object Identifier (DOI). Nearly all the papers or preprints you read or produce have one. The DOI serves as...publications help to enable reproducibility of experiments. Additionally, proper citation of these Addgene...standardized persistent identification of research reagents, several journals or organizations now recommend... the RRID and compact identifier formats. Acknowledgments Many thanks to Daniela Bourges for editing ...

...that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized tracking...and developments.  MTAs also mean that everyone is on the same page as to how the shared reagents should...organizations to make policy decisions on research use agreements. The UBMTA, which is the MTA most plasmids at...department is responsible for managing research use agreements like MTAs.  Addgene does a lot to ensure that...tracking when biological reagents are shared — both at what time and with whom. Tracking these connections...

...in silico. These algorithms are trained on vast amounts of data to calculate the relative immunogenicity... replicate the complexity of antigen processing events that take place within an antigen-presenting cell...conditions, etc. that can vary widely between recipients.   In vitro prediction of drug immunogenicity... to validate the findings from the in silico assessments, as well as analyze the contributing factors ...in-vitro models can be built that contain various components of the immune system. Figure 2: In-vitro...Potential Use of HLA Haplotypes to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org...Algorithm Infrastructure and a Vision for Future Enhancements. Frontiers in drug discovery, 2, 952326. https...

...classes, workshops, book clubs, seminars, and social events. Building a community science lab In the Fall...it. We started out by holding a few journal club events and soon realized that it was easy to find people...entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using nanopore ...sequencing at BosLab to perform proof-of-concept experiments for discovering novel therapeutics. Dave is a...optimizes plant cell culture and transgenesis he wants to share his knowledge with the community by teaching... will engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly...ecology but she became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast ...

...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing... bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells...transfection. Unlike bacterial transformation, scientsts do not "select" for cells that have taken up the...transfection and the elusive "mammalian ORI" For many experiments, it is sufficient for the transfected plasmid...plasmid replicates in the cell, as many of these experiments are concluded within 48 h of transfection. Of...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...

...their required components are very similar. While plasmids containing virus-specific elements such as the...or infrequently cycling cells but is fine for experiments with immortalized cell line cultures. Lentivirus...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...whole animal administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally...strategies may need to be considered if size constraints are an issue. Adenovirus Adenovirus has a linear...Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral...

...competitive endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a... to the opportunities of their dream.  To help students focus on the application process, and simplify...internships for high school, college, and graduate students. In order to gain a competitive advantage this...errors, sentence structures, and overall format (fonts, font size, legibility, and more). Tips on drafting...leadership status in their current field of expertise augments their authoritative voice. Send a draft of your...in the Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...outreach among low-income communities to educate parents about resources that can help their children thrive...

... but should contain a table of contents that lays out which experiments can be found in each folder. This...their results. We are adept at recording wet-lab experiments using a “traditional” lab notebook; however, ...the future. Stay organized Set up a table of contents in the top directory: A directory is a file system...document with a brief description of the directory contents, at a minimum. This may or may not be where you...in tact! Take notes Comment in your scripts: Comments on what a given line of code does are very useful...Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional...