We narrowed to 599 results for: REV;
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TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m.... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall...
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Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set...related versions that have different properties. To prevent misidentification, we augment the database with... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....cell via endocytosis. In our study, we aimed to prevent the uptake of AAV2 viral particles by our packaging... cells with the endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated that the ... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....other hand, if it opens up an epitope that was previously hidden, an antibody might suddenly work that ...denatured, allowing the antibody to bind to a previously hidden epitope (right). Created withBioRender.com... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m.... are also of practical interest since they’ve previously yielded valuable enzymes. Famously, Taq polymerase...PNAS. Two other thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....CRISPR construct needs to be segregated away to prevent continued production of new alleles or off-target... generation when CRISPR needs to be removed to prevent further editing, then green offspring can be selected... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were discussed... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....context, it’s hypothesized that base removal helps prevent the invading genome from “recovering” its original...cannot switch guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m.... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535. Lederberg...Bacterial Plasmids: A Proposal.”Bacteriological Reviews 40.1 (1976): 168–189. PubMed PMID: 16350226. PubMed...