We narrowed to 807 results for: APP

1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...

2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ... markers make it easy to spot flies with the appropriate transgene. One such marker is the white gene,...

3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   Blog Post

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   July 6, 2017, 1:59 p.m.

   ...interactions, cancer immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation...

4. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction ...

5. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

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   March 30, 2017, 2:30 p.m.

   ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

6. Building and Validating Recombinant Antibodies: A Case Study

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   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1 subclass (Manning...

7. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

8. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...discussed in this post, voltage has been adjusted appropriately and compensation performed. Before you read ...

9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...

10. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

12. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...relationships that are memorable. It isn’t always appropriate to walk up to someone much more senior than you...

13. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ... cells to the microenvironment. This mechanism happens for both short and long distances. When it comes...

14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...take place in a tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain...

15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

16. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...

17. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

18. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...densely populated areas. This assumption does not appear to hold. There are institutions in Boston - Massachusetts...

19. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ... glycerol stocks, so make sure to select the appropriate service and read the submission instructions....

20. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...BEST) awards, which are designed to support new approaches that address the need to train our young scientists...

21. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

22. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...Industry Professionals”. This larger shop might appeal to some although most scientist coaches can help...

23. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...choose essentially any background, but two common approaches are either a solid color, or a “themed” background...

24. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...influence (non-homologous end joining, or NHEJ), in approximately one percent of cases, the stem cell will seek...

25. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

26. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ... also used to teach students about restriction mapping and western blotting. Students who participate ...

27. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later...

28. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody pairs. Because the...

29. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

30. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can...