We narrowed to 525 results for: ARL
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TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m.... and a few proteases knocked out making them particularly useful for protein production), then dilute ...
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Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....orange fluorescent protein, CyOFP1. CyOFP1 was molecularly evolved from mNeptune2 through 33 mutations and... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m.... a type of pediatric kidney cancer. This was particularly interesting as I had just completed my thesis... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m.... or excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....receptors (i.e. chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....Medicine at the University of Washington. She is particularly interested in science communication and in vitro... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....the “elegans” methods outlined in this post – particularly the use of co-selection methods – could greatly... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....about 90% of identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....spectrometry to identify the interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....tissues of this size. For example, it could take nearly two months to image an entire mouse brain with ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m.... studied cellular behavior. This approach is particularly suitable for in vivo screens, where CRISPR modified... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....leading to dimmer fluorescence. In fact, many of the early reversible PA-FPs also display this “negative” photoswitching... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....stable signal over extended periods, which is particularly beneficial when studying dynamic processes like... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m.... Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...