We narrowed to 582 results for: BASE;

...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

...application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead...

...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

...followed by sequencing and further data analysis. Based on Figure 31.5.2 from https://pubmed.ncbi.nlm.nih.gov...

...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes...

...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection...

...together a really great solution that covered all the bases. You may have also used your writing skills to convey...

...you do? What follows are just a few suggestions based on what I have found to be especially beneficial...

...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

... it, could allow idea generation and extension, based on existing, published data. The importance of data...

... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

...discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss...