We narrowed to 575 results for: BASE;
-
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....resources towards the development of a new therapy based on its market need and anticipated commercial success...
-
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....together a really great solution that covered all the bases. You may have also used your writing skills to convey... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....you do? What follows are just a few suggestions based on what I have found to be especially beneficial... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m.... it, could allow idea generation and extension, based on existing, published data. The importance of data... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m.... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m.... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....reporters have limited use in vivo because they are based on light which is very easily scattered by tissue... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....representing ~85% of the postdoctoral workforce. Based on our communications with and research on these... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....could reveal something fascinating. Behavior is based primarily on careful observation of what an animal... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m.... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m.... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,...