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TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...
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Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn them on or off...News & Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First,... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m....collect in those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental...Then, they use this material to produce various grades of plastic lumber and distribute it to industrial... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....on the Addgene blog and have created and updated guides on various topics in biology over the past year...containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....choice picks this month, I’m picking two posts that describe methods to make single base edits in DNA. The ...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met at a lindy...to possess the correct phages will test. These includes labs with a large number of different phages or... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....LaFleur, et al. 2024. The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer...blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....interactive game that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you...all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the changes at... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....are the three main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector...AAV from our viral service can share experimental design and data. With these different approaches to open... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....new Help Center! Created using the same reliable Zendesk system that powers our email support, this portal...your name and email address, after which you can describe your issue and press send. Doing so will bring... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....Improving Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m.... favors the breakage of the hydrogen bonds, destabilizing the molecule and favoring the separation of ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....optimized for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then arg1 vesicles... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....when a high degree of signal to noise is desired. It also provides an excellent additional measure of DNA... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....repository and serving scientists better. That includes keeping fees as low as possible to ensure that...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m.... Being an SME is often like being a jack-of-all-trades. “As a subject matter expert in the educational...the courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also...