We narrowed to 538 results for: EPO;

1. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide...

3. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

4. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

5. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...proteins. Here, we’ll highlight a few recently-deposited optogenetic tools to control the cytoskeleton ...

7. Transferable Skills Guide: Time Management

   Type

   Blog Post

   Published

   Dec. 5, 2017, 1:52 p.m.

   ... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly...

8. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...repurposed for a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the...

9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

10. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...growth as RNA biology, we are looking forward to reports on the next additions to aptamer soup! References...

11. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064...

13. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...Immigrants National Science Foundation: Universities Report Fourth Straight Year of Declining Federal R&...

14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ... the Patron Lab.  As with all plasmids in the repository, we highly recommend reading the associated publications...

15. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins...

16. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated serine at the TAG...

17. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...already commercially available although they are reported to have high error rates (10). It is projected...

18. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...drop down menu of common cloning options in the deposit process in 2014 because of its gain in popularity...

19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases...

20. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...(the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase Knowledge...