We narrowed to 776 results for: IRE;

...smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and file ... to the necessary time and resource investment required to change the status quo. But with recent improvements...

... been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

...is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed for one dCas9...fluorescently labeled dCas9 isoforms are efficiently directed to the proper target sequence. They were also ...

...RNAi, the field for which Craig Mello and Andrew Fire won the Nobel Prize. What did Dr. Doudna think was...gRNA and crRNA could be combined into one RNA to direct cleavage the result was a simpler 1 protein - 1...

...These apurinic sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations...concentration. Alternatively, if the priority is to directly use your DNA preparation in any downstream application...

..., and, as mentioned earlier, how frequently the entire group will meet. You and your collaborators should...with everyone involved can speed up the future directions of the project. The state of research is constantly...

...only makes CRISPR screen results available to the entire biomedical research community, but also allows ...identifier, which can also be used to search the entire CRISPR database for particular screens of interest...

... GC-rich and repetitive regions. These regions require additional verification experiments that can be...of Snapgene. When you download a Snapgene file directly from our website, you can open it with either ...

...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...gene The rep gene encodes four proteins that are required for viral genome replication and packaging: Rep78...

...companies have the potential to make a huge impact directly on waste, but also on culture. Encourage your ...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...

...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...

...2 components: 1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon...Beauty transposase as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is...

...long, skinny things") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort...Montana! Though sadly I will not be attending the entire meeting, Monday's talks alone were worth the trip...

...over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop buffer...commercial boxes were small enough to minimize the required area and buffer volume.  So, I designed my own...

...survive. Genome engineering and future directions CRISPR/Cas inspired genome editing was initially described...

...turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about our paper...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

...antibody tool and therefore will grow to serve the entire scientific community.     Why recombinant antibodies.... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ...

...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

...and control elements. Development of this tool required creating a database of common features, and devising...Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity when detecting a...